Ancient DNA Typing

Ancient DNA Typing

Einband:
Kartonierter Einband
EAN:
9783642077050
Untertitel:
Methods, Strategies and Applications
Genre:
Sozialwissenschaften, Recht & Wirtschaft
Autor:
Susanne Hummel
Herausgeber:
Springer Berlin Heidelberg
Auflage:
Softcover reprint of hardcover 1st ed. 2003
Anzahl Seiten:
312
Erscheinungsdatum:
09.12.2010
ISBN:
3642077056

Ancient DNA Typing is a comprehensive manual about the analysis of ancient and degraded DNA. It is an equally useful textbook and working tool for various disciplines such as: Anthropology, Archaeology, Forensic Medicine, Conservation Biology, Environmental Sciences and Food Engineering.
The reader benefits from a clear division of strategies, methods and applications with many interesting examples of the analysis of historic skeletal and cultural remains. The book contains a detailed protocol chapter which serves as a lab manual with the most important methods for DNA extraction, PCR and electrophoretic analysis of degraded samples. The appendix helps to set up a PCR lab specializing in ancient DNA research and answers the most frequent questions concerning technical problems in investigations of degraded DNA.

Source of information on techniques to identify and sequence old DNA and on their use in biological and medical research and application

Klappentext
Ancient DNA Typing is a comprehensive manual about the analysis of ancient and degraded DNA. It is an equally useful textbook and working tool for various disciplines such as: Anthropology, Archaeology, Forensic Medicine, Conservation Biology, Environmental Sciences and Food Engineering. The reader benefits from a clear division of strategies, methods and applications with many interesting examples of the analysis of historic skeletal and cultural remains. The book contains a detailed protocol chapter which serves as a lab manual with the most important methods for DNA extraction, PCR and electrophoretic analysis of degraded samples. The appendix helps to set up a PCR lab specializing in ancient DNA research and answers the most frequent questions concerning technical problems in investigations of degraded DNA.

Zusammenfassung
Ancient DNA Typing is a comprehensive manual about the analysis of ancient and degraded DNA. It is an equally useful textbook and working tool for various disciplines such as: Anthropology, Archaeology, Forensic Medicine, Conservation Biology, Environmental Sciences and Food Engineering.
The reader benefits from a clear division of strategies, methods and applications with many interesting examples of the analysis of historic skeletal and cultural remains. The book contains a detailed protocol chapter which serves as a lab manual with the most important methods for DNA extraction, PCR and electrophoretic analysis of degraded samples. The appendix helps to set up a PCR lab specializing in ancient DNA research and answers the most frequent questions concerning technical problems in investigations of degraded DNA.

Inhalt
1 Introduction.- 2 DNA markers.- 3 aDNA extraction.- 4 PCR.- 5 Analysis of PCR products.- 6 Authenticity of results.- 7 Applications.- 8 Protocols.- I Laboratory equipment.- I.1 Pre-PCR laboratory equipment.- I.2 PCR laboratory.- I.3 Post-PCR laboratory.- I.4 Photographic documentation.- II PCR trouble shooting.- III Internet sites of interest.


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