Handbook of Fluorescence Spectroscopy and Imaging

Providing much-needed information on fluorescence spectroscopy and microscopy, this ready reference covers detection techniques, data registration, and the use of spectroscopic tools, as well as new techniques for improving the resolution of optical microscopy below the resolution gap.

Autorentext
Markus Sauer studied chemistry in Karlsruhe, Saarbrücken, and Heidelberg, and finished his PhD in Physical Chemistry at the University of Heidelberg under the guidance of Prof. Jürgen Wolfrum. After a short-term visit at LBNL, Berkeley in the group of Prof. Shimon Weiss, he was decorated with the BioFuture award to perform independent research on single-molecule handling, detection, and identification. He finished his habilitation in Heidelberg at the Institute of Physical Chemistry and is now Professor for Applied Laser Physics and Laser Spectroscopy at the University in Bielefeld. Johan Hofkens received his MSc. (1988) and Ph.D. degree (1993) in Chemistry from the University of Leuven (K.U.Leuven). After postdoctoral research with Prof. Masuhara at Osaka University and Prof. Barbara at the University of Minneapolis, he rejoined the K.U.Leuven supervising the Single Molecule Unit in the group of Prof. De Schryver. In 2005 he was appointed Research Professor at the K.U.Leuven and recently he was promoted to full professor. His research interests are fast spectroscopy, (single molecule) fluorescence microscopy and nanoscopy and the application of these techniques in material science and biosciences.

Klappentext
Jörg Enderleins studied physics at the Mechnikov University in Odessa (Ukraine) from 1981 until 1986, and defended his PhD thesis about non-linear reaction diffusion system at Humboldt University in berlin (Germany) in 1991. From 1991 until 1996 he was scientific co-worker of PicoQuant GmbH, where he was involved in the development of advanced single-photon counting technology for single-molecule fluorescence spectroscopy. From 1996 until 1997, he joined the group of Richard A. Keller in Los Alamos (USA) as a guest scientist, and in 1997 became an assistant professor at the University of Regensburg (Germany), where he defended his habilitation in 2000. From 2001 until 2006, he was as Heisenberg Fellow of the German Research Council (DFG) and established his research group at the Insitut for Biological Information processing 1 at the Forschungszentrum Jülich (Germany). His main research topic is the development of new single-molecule spectroscopic techniques for biophysics applications. Johan Hofkens, born in Hoogstraten, Belgium, in 1966 received his master in Chemistry from KUleuven in 1988, which was followed by a PhD in Sciences from KULeuven in 1993. After postdoctoral research with professor Masuahara at Osaka University and Professor Barbara at the University of Minneapolis, he rejoined the KULeuven were he was in charge of the single molecule unit in the group of Prof De Schryver. In 2005 he was appointed research professor at the KULeuven. His research interests are fast spectroscopy, single molecule spectroscopy and optics. Markus Sauer was born 1965 in Pforzheim. He studied chemistry in Karlsruhe, Saarbrücken, and Heidelberg, and finished his PhD in Physical Chemistry at the University of Heidelberg in 1995 under the guidance of Prof. Jürgen Wolfrum. After a short-term visit at LBNL, Berkeley in the group of Prof. Shimon Weiss, he was decorated with the BioFuture award in 1998 to perform independent research on single-molecule handling, detection, and identification. 2002 he finished his habilitation in Heidelberg at the Institute of Physical Chemistry. Since 2003 he is Professor for Applied Laser Physics and Laser Spectroscopy at the University in Bielefeld. His research interests cover the development of new electron transfer sensors and probes as well as new single-molecule sensitive fluorescence spectroscopic techniques. Kenneth Weston was born and grew up in Winston-Salem, North Carolina. After receiving a BS degree in Chemistry from the University of North Carolina, Chapel Hill in 1994, he went on to earn a PhD in Chemistry from the University of California, Santa Barbara in 1998 where he worked with Prof. Steve Buratto. Kenneth spent two years working with Dr. Lori Goldner at The National Institute of Standards and Technology in Gaithersburg, MD as a National Research Council Postdoctoral Associate. In 2001, Kenneth joined to group of Dr. Markus Sauer at the Physical Chemistry Institute at the University of Heidelberg in Germany. From 2002-2005, Kenneth was an Assistant Professor in the Department of Chemistry and Biochemistry at Florida State University. Kenneth's research has focused on the development of various new methods and applications of optical spectroscopy and microscopy, fluctuation correlation spectroscopy, and microfluidics. Kenneth is now a senior scientist with Directed Energy Solutions in Colorado Springs and is part of a team that is developing very high power infrared lasers.

Inhalt
Basic Principles of fluorescence spectroscopy Fluorophores and Fluorescent Labels Fluorophore Labeling for Single-Molecule Fluorescence Spectroscopy (SMFS) Fluorophore Selection for Single-Molecule Fluorescence Spectroscopy (SMFS) and Photobleaching Pathways Fluorescence Correlation Spectroscopy Excited state energy transfer Photoinduced Electron Transfer (PET) Reactions Super-Resolution Fluorescence Imaging Single-molecule enzymatics

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