Flim Microscopy in Biology and Medicine

Zusatztext "?this book represents a clear presentation of the theory! instrumentation! and data analysis of FLI microscopy and is enhanced by the critical discussions of assumptions! limitations and confounding elements? ."-Barry R. Masters! MIT! Microscopy and Microanalysis"?an excellent and comprehensive treatment of FRET in biology investigation. It has a good balance of basic theory and cutting-edge development in both techniques and applications."-Peter So! Ph.D.! MIT"An amazingly comprehensive reference-I can't imagine anyone looking for information there and not finding what they need. It is quite essential for any lab doing FLIM."-Guy Cox! University of Sydney! Australia Informationen zum Autor Ammasi Periasamy, University of Virginia, Charlottesville, USA Robert M. Clegg, University of Illinois, Urbana-Champaign, USA Klappentext Suitable for a variety of applications in biology and biomedical research, fluorescence lifetime imaging (FLIM) is a powerful new tool for advanced quantitative analysis. Its high contrast and sensitivity allow for the detection of signals at the single molecule level. After an overview of basic principles of fluorescence, microscopy, and FLIM, this book discusses probes, dynamic and video rate FLIM imaging, laser scanning confocal FLIM, spectral lifetime imaging, nonlinear curve-fitting methods, global FLIM data analysis, and new approaches in data analysis as well as current applications. Zusammenfassung Presents an overview of basic principles of fluorescence, microscopy, and fluorescence lifetime imaging (FLIM). This book discusses probes, dynamic and video rate FLIM imaging, laser scanning confocal FLIM, spectral lifetime imaging, nonlinear curve-fitting methods, global FLIM data analysis, and fresh approaches in data analysis. Inhaltsverzeichnis Basics of Fluorescence. Basics of Microscopy. Basics of FLIM Microscopy. Probes for FLIM Microscopy. Dynamic FLIM Microscopy Imaging Using Gating Camera (Time Domain). Video Rate FLIM Imaging Microscopy (Frequency Domain). Laser Scanning Confocal FLIM Microscopy. Multiphoton-Excitation Time-Correlated Single-Photon Counting FLIM Microscopy. SLIM for Multispectral Imaging Microscopy. Nonlinear Curve-Fitting Methods for FLIM Data Analysis. Global FLIM Data Analysis. New Approaches in FLIM Data Analysis: Polar Plot. Critical Review of FLIM Applications. ...

"this book represents a clear presentation of the theory, instrumentation, and data analysis of FLI microscopy and is enhanced by the critical discussions of assumptions, limitations and confounding elements ."Barry R. Masters, MIT, Microscopy and Microanalysis "an excellent and comprehensive treatment of FRET in biology investigation. It has a good balance of basic theory and cutting-edge development in both techniques and applications."Peter So, Ph.D., MIT "An amazingly comprehensive referenceI can't imagine anyone looking for information there and not finding what they need. It is quite essential for any lab doing FLIM."Guy Cox, University of Sydney, Australia

Autorentext
Ammasi Periasamy, University of Virginia, Charlottesville, USARobert M. Clegg, University of Illinois, Urbana-Champaign, USA

Klappentext
Suitable for a variety of applications in biology and biomedical research, fluorescence lifetime imaging (FLIM) is a powerful new tool for advanced quantitative analysis. Its high contrast and sensitivity allow for the detection of signals at the single molecule level. After an overview of basic principles of fluorescence, microscopy, and FLIM, this book discusses probes, dynamic and video rate FLIM imaging, laser scanning confocal FLIM, spectral lifetime imaging, nonlinear curve-fitting methods, global FLIM data analysis, and new approaches in data analysis as well as current applications.

Zusammenfassung
Presents an overview of basic principles of fluorescence, microscopy, and fluorescence lifetime imaging (FLIM). This book discusses probes, dynamic and video rate FLIM imaging, laser scanning confocal FLIM, spectral lifetime imaging, nonlinear curve-fitting methods, global FLIM data analysis, and fresh approaches in data analysis.

Inhalt
Basics of Fluorescence. Basics of Microscopy. Basics of FLIM Microscopy. Probes for FLIM Microscopy. Dynamic FLIM Microscopy Imaging Using Gating Camera (Time Domain). Video Rate FLIM Imaging Microscopy (Frequency Domain). Laser Scanning Confocal FLIM Microscopy. Multiphoton-Excitation Time-Correlated Single-Photon Counting FLIM Microscopy. SLIM for Multispectral Imaging Microscopy. Nonlinear Curve-Fitting Methods for FLIM Data Analysis. Global FLIM Data Analysis. New Approaches in FLIM Data Analysis: Polar Plot. Critical Review of FLIM Applications.

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