Protein Analysis and Purification

Fully revised and newly enhanced, PROTEIN ANALYSIS AND PURIFICATION provides the research with the most complete picture of the state of the art technology and this expanding and dynamic field. Now enhanced with data on mass spectrometry techniques, the use of robotics and in silicon science, and in appendix of online databases, this new edition provides the most current, complete, and user-friendly bench side resource to both the experienced research and the novice investigator.

Each chapter contains a wealth of practical information, including troubleshooting tips, safety icons and references More than 180 protocols included with materials lists, step-by-step instructions and valuable comments on practical issues such as time considerations and safety issues Includes supplementary material: sn.pub/extras

Klappentext
New technologies and the manner in which scientific research is conducted have been fully incorporated into this thoroughly revised second edition of PROTEIN ANALYSIS AND PURIFICATION. Among the many topics introduced to this new edition are the use of robotics in the lab, the impact of the codification of the human genome, functional proteomics, mass spectrometry and the expanding use of bioinformatics as a tool to improve efficiency and productivity. The author has also included an annotated appendix of the educational materials and technical databases available online to enhance the researcher's benchside access to the latest resources. With solid explanations of the science asking the "why" and literally dozens of step-by-step protocols providing the "how", this new edition is a complete resource for the benchside.  

Inhalt
An Overview of This Manual.- Protein Structure.- Tracking the Target Protein.- Electrophoretic Techniques.- Getting Started with Protein Purification.- Membrane Proteins.- Transfer and Detection of Proteins on Membrane Supports.- Identification of the Target Protein.- Identifying and Analyzing Posttranslational Modifications.- Chromatography.- Recombinant Protein Techniques.

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