Green Fluorescent Protein

Informationen zum Autor MARTIN CHALFIE, PhD , is William R. Kenan Jr. Professor of Biological Sciences at Columbia University. He is world renowned for introducing the use of GFP as a biological marker. A member of the National Academy of Sciences, Dr. Chalfie serves on the editorial boards of Genome Biology and Molecular Biology of the Cell. STEVEN R. KAIN, PhD , manages the Applications Gene Expression Group for Agilent Technologies. While director of cell biology at Clontech Laboratories, he was responsible for commercializing the first products based on GFP and produced the popular EGFP variant. He has twelve years' experience with a variety of biotechnology firms in the San Francisco Bay Area. Klappentext Eigenschaften und Anwendungen von nicht fluoreszierenden und von grün fluoreszierenden Proteinen (NFPs bzw. GFPs) stehen im Mittelpunkt dieses Bandes, der auf solider theoretischer Grundlage Einsatzgebiete in der Industrie (etwa in der Biosensorentechnik) diskutiert. Die Beiträge, von ausgewiesenen Experten verfasst, beschreiben das maßgeschneiderte Design von GFPs und NFPs für spezifische Systeme, die Maximierung der Expression, Maßnahmen zur Verbesserung der Detektion und andere interessante Themen. Neben vielen praktischen Hinweisen erhalten Sie auch Tipps zum Vermeiden häufiger Fehler. Zusammenfassung Since the discovery of the gene for green fluorescent protein (GFP), derived from jellyfish, this protein that emits a green glow has initiated a revolution in molecular biosciences. With this tool, it is now possible to visualize nearly any protein of interest in any cell or tissue of any species.

Autorentext
MARTIN CHALFIE, PhD, is William R. Kenan Jr. Professor of Biological Sciences at Columbia University. He is world renowned for introducing the use of GFP as a biological marker. A member of the National Academy of Sciences, Dr. Chalfie serves on the editorial boards of Genome Biology and Molecular Biology of the Cell. STEVEN R. KAIN, PhD, manages the Applications Gene Expression Group for Agilent Technologies. While director of cell biology at Clontech Laboratories, he was responsible for commercializing the first products based on GFP and produced the popular EGFP variant. He has twelve years' experience with a variety of biotechnology firms in the San Francisco Bay Area.

Klappentext
Eigenschaften und Anwendungen von nicht fluoreszierenden und von grün fluoreszierenden Proteinen (NFPs bzw. GFPs) stehen im Mittelpunkt dieses Bandes, der auf solider theoretischer Grundlage Einsatzgebiete in der Industrie (etwa in der Biosensorentechnik) diskutiert. Die Beiträge, von ausgewiesenen Experten verfasst, beschreiben das maßgeschneiderte Design von GFPs und NFPs für spezifische Systeme, die Maximierung der Expression, Maßnahmen zur Verbesserung der Detektion und andere interessante Themen. Neben vielen praktischen Hinweisen erhalten Sie auch Tipps zum Vermeiden häufiger Fehler.

Zusammenfassung
Since the discovery of the gene for green fluorescent protein (GFP), derived from jellyfish, this protein that emits a green glow has initiated a revolution in molecular biosciences. With this tool, it is now possible to visualize nearly any protein of interest in any cell or tissue of any species.

Inhalt
First Edition Preface.Preface.Contributors.1. Discovery of Green Fluorescent Protein (Osamu Shimomura).2. Photons for Reporting Molecular Events: Green Fluorescent Protein and Four Luciferase Systems (J. Woodland Hastings and James G. Morin).3. Biochemical and Physical Properties opf Green Fluorescent Protein (William W. Ward).4. The Three-Dimensional Structure of Green Fluorescent Protein and Its Implications for Function and Design (George N. Phillips).5. Molecular Biology and Mutation of Green Fluorescent Protein (David A. Zacharias and Roger Y. Tsien).6. Discovery and Properties of GFP-Like Proteins from Nonbioluminescent Anthozoa (Konstantin A. Lukyanov, Dmitry M. Chudakov, Arkady F. Fradkov, Yulii A. Labas, Mikhail V. Matz, and Sergey Lukyanov).7. Evolution of Function and Color in GPF-Like Proteins (Mikhail V. Matz, Yulii A. Labas, and Juan Ugalde).8. The Uses of Green Fluorescent Protein in Prokaryotes (Raphael H. Valdivia, Brendan P. Cormack, and Stanley Falkow).9. The uses of Green Fluorescent Protein in Yeasts (Amy L. Hitchcock, Jason A. Kahana, and Pamela A. Silver).10. Uses of GFP in Caenorhabditis Elegans (Oliver Hobert and Paula Loria).11. Green Fluorescent Protein Applications in Drosophila (Tulle Hazelrigg and Jennifer H. Mansfield).12.The Uses of Green Fluorescent Protein in Plants (Jim Haseloff and Kirby R. Siemering).13. Uses of GFP in Transgenic Vertebrates (Sean Magason, Adam Amsterdam, Nancy Hopkins, and Shuo Lin).14. The Uses of Green Fluorescent Protein in Mammalian Cells (Theresa H. Ward and Jennifer Lippincott-Schwartz).15. Practical Considerations for Use of Reef Coral Fluorescent Proteins in Mammalian Cells: Applications in Fluorescent Microscopy and Flow Cytometry (Yu Fang, Olivier DA(c)ry, Michael Haugwitz, Pierre Turpin, and Steven R. Kain).16. Pharmaceutical Applications of GFP and RCFP (Nicola Bevan and Stephen Rees).17. Reassembled GFP: Detecting Protein-Protein Interactions and Protein Expression Patterns (Thomas J. Magliery and Lynne Regan).Methods and Protocols (Steven R. Kain).Index.

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