Cryo-EM Part B: 3-D Reconstruction

This volume is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied.* Includes time-tested core methods and new innovations applicable to any researcher * Methods included are useful to both established researchers and newcomers to the field * Relevant background and reference information given for procedures can be used as a guide

Inhalt
Fundamentals of three-dimensional reconstruction from projections Pawel A. Penczek Image Restoration in Cryo-electron Microscopy Pawel A. Penczek Resolution measures in molecular electron microscopy Pawel A. Penczek 3D reconstruction from 2D crystal image and diffraction data Andreas Schenk, Daniel Castaño-Díez, Bryant Gipson, Marcel Arheit, Xiangyan Zeng, and Henning Stahlberg Fourier-Bessel Reconstruction of Helical Assemblies David L Stokes, Ruben Diaz & William, J. Rice Reconstruction of Helical Filaments and Tubes Edward H Egelman Three-Dimensional Asymmetric Reconstruction of Tailed Bacteriophage Jinghua Tang, Robert S. Sinkovits, and Timothy S. Baker Single Particle Analysis at High Resolution Yao Cong and Steven J. Ludtke The Orthogonal Tilt Reconstruction method Andres Leschziner An introduction to maximum-likelihood methods in cryo-EM Fred J. Sigworth, Peter C. Doerschuk, Jose-Maria Carazo, and Sjors H.W. Scheres Classification of structural heterogeneity by maximum-likelihood methods Sjors H.W. Scheres Methods for three-dimensional reconstruction of heterogeneous assemblies Elena V Orlova, Helen R. Saibil Alignment of cryo-electron tomography datasets Fernando Amat, Daniel Casta~no-Diez, Albert Lawrence, Farshid Moussavi, Hanspeter Winkler, & Mark Horowitz Correcting for the Ewald sphere in high resolution single particle reconstructions Grant J Jensen, Peter A. Leong, Xuekui Yu, & Z. Hong Zhou Software Tools For Molecular Microscopy: An Open-Text Wikibook Bridget Carragher, Neil R. Voss, Clinton S. Potter, & Ross Smith

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