Calcium Signaling

Informationen zum Autor James W. Putney Jr. Klappentext This fully revised edition focuses on those processes that generate calcium signals! to offer the first comprehensive exploration of calcium signaling research from a methodological standpoint. Several new chapters have been added and in many cases! where chapters from the first edition were retained! new experts were recruited to offer a fresh perspective. Employing a comfortable format! this volume offers numerous research techniques! along with the pros and cons of each. This work contains invaluable information for established researchers! as well as for those graduate students and scientists just beginning to find a direction in cellular calcium signaling. Zusammenfassung Deals with methods for studying calcium from a variety of perspectives. This work discusses calcium indicators and other tools, and looks at microscopic and electrophysiological techniques, as well as other special methodological aspects of calcium signaling research. Inhaltsverzeichnis Fluorescent Calcium Indicators Based on BAPTA; Martin Poenie Fluorescent Indicators-Facts and Artifacts; Gary St. J. Bird and James W. Putney Jr. Fluorescence Microplate-Based Techniques for the High-Throughput Assessment of Calcium Signaling: The Highs and Lows for Calcium Researchers; Gregory R. Monteith and Gary St. J. Bird Genetically Encoded Fluorescent Calcium Indicator Proteins; Atsushi Miyawaki! Takeharu Nagai! and Hideaki Mizuno Targeted Aequorins; Andrea Prandini and Rosario Rizutto Electrophysiological Recordings of Ca2? Currents; Anant B. Parekh Molecular Biology of Ca2? Channels: Lessons from the TRP Superfamily; Veit Flockerzi! Thomas Aberle! Marcel Meissner! Christine Jung! Stephan Philipp! and Ulrich Wissenbach Genetic and Molecular Characterization of Ca2? and IP3 Signaling in the Nematode Caenorhabditis elegans; Ana Y. Estevez and Kevin Strange Calcium Signaling Networks in Yeast; Kyle W. Cunningham Nuclear Patch Clamp Electrophysiology of Inositol Trisphosphate Receptor Ca2? Release Channels; Don-On Daniel Mak! Carl White! Lucian Ionescu! and J. Kevin Foskett Ryanodine Receptors; Xander H.T. Wehrens! Stephan E. Lehnart! and Andrew R. Marks Methods in Cyclic ADP-Ribose and NAADP Research; Anthony J. Morgan! Grant C. Churchill! Roser Masgrau! Margarida Ruas! Lianne C. Davis! Richard A. Billington! Sandip Patel! Michiko Yamasaki! Justyn M. Thomas! Armando A. Genazzani! and Antony Galione Methods for Studying Calcium Pumps; Leonard Dode! Luc Raeymaekers! Ludwig Missiaen! Bente Vilsen! Jens P. Andersen! and Frank Wuytack Measuring Single Cell and Subcellular Ca2? Signals; Sandip Patel! Lawrence D. Gaspers! Nicola Pierobon! Walson Metzger! and Andrew P. Thomas Subcellular Compartmentalization of Calcium Signaling; Nicholas J. Dolman! Michael C. Ashby! Myoung K. Park! Ole H. Petersen! Alexei V. Tepikin Apoptosis; Clark W. Distelhorst Calcium Entry Channels and Drug Discovery; Su Li! Anne Dodge! Chris T. Poll! and Martin Gosling Index ...

Autorentext
James W. Putney Jr.

Klappentext
This fully revised edition focuses on those processes that generate calcium signals, to offer the first comprehensive exploration of calcium signaling research from a methodological standpoint. Several new chapters have been added and in many cases, where chapters from the first edition were retained, new experts were recruited to offer a fresh perspective. Employing a comfortable format, this volume offers numerous research techniques, along with the pros and cons of each. This work contains invaluable information for established researchers, as well as for those graduate students and scientists just beginning to find a direction in cellular calcium signaling.


Zusammenfassung
Deals with methods for studying calcium from a variety of perspectives. This work discusses calcium indicators and other tools, and looks at microscopic and electrophysiological techniques, as well as other special methodological aspects of calcium signaling research.

Inhalt
Tools and Techniques. Comparative and Genetically Tractable Systems. Cellular and Molecular Basis for Calcium Signaling.

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