DNA Fingerprinting in Plants

Zusatztext "this is a good book and a useful addition to the shelf. It should be in the library (or preferably the lab) of all institutes carrying out DNA fingerprinting in plants. Its reference list is extensive! and it is a source of a wide range of interesting pieces of information. The different expertise of the authors has resulted in a wide-ranging and detailed assessment of the subject. The authors should be congratulated on the production of a good successor to the first edition of the book."--Michelle Hollingsworth in the Annals of Botany"? many readers will find this book interesting. ? It is analytically written and contains much valuable information. It involves perhaps all published plant DNA isolation protocols! a brief description of them! and notes on their uses. ? the book contains a plethora of information on plant DNA fingerprinting."- Elias Anastassopoulos! Tei Larissas! School of Agriculture! Dep. Of Plant Production! Larissa! Greece in Economic Botany 61(1) 2007 Informationen zum Autor Kurt Weising, Hilde Nybom, Markus Pfenninger, Kirsten Wolff, Günter Kahl Klappentext Like its successful predecessor, this completely revised second edition serves as a comprehensive introduction to the field, as well as a handy reference for the researcher. This edition focuses on both PCR-based fingerprinting and more sophisticated techniques. Supplying relevant background information, it provides a complete update of the extensive survey of applications of DNA fingerprinting in plants and a comparison of different types of molecular markers. This second edition is a valuable manual for beginners and active researchers. Zusammenfassung Given the explosive development of new molecular marker techniques over the last decade, newcomers and experts alike in the field of DNA fingerprinting will find an easy-to-follow guide to the multitude of techniques available in DNA Fingerprinting in Plants: Principles, Methods, and Applications, Second Edition. Along with step-by-step annotated protocols, the authors fully discuss the technical aspects and modifications of existing techniques, the influence of reaction components and conditions, and the analysis of the results. This second edition has been completely revised to address the exponential changes in the field since the first edition, focusing on PCR-based techniques but also including more sophisticated ones. The authors include numerous case studies to illustrate applications of the methods, more than 1600 references to the literature, and descriptions of reagent formulation, equipment, and computer programs used for evaluating molecular marker data. They compare the various methods, including the costs and benefits of each, helping readers determine which is best suited to a particular application. The well-rounded, cross-referenced, and unified nature of this book makes it intrinsically easier to follow than the edited, multi-authored books currently available. It is an absolute necessity on the lab bench of anyone involved in plant research, DNA profiling, and molecular markers. Inhaltsverzeichnis Preface. Repetitive DNA: An Important Source of Variation in Eukaryotic Genomes. Detecting DNA Variation by Molecular Markers. Laboratory Equipment. Methodology. Evaluation of Molecular Marker Data. Applications of DNA Fingerprinting in Plant Sciences. Linkage Analysis and Genetic Maps. Which Marker for What Purpose: A Comparison. Future Prospects: SNiPs and Chips for DNA and RNA Profiling. Appendix 1: Plant DNA Isolation Protocols. Appendix 2: Suppliers and Sellers of Reagents and Equipment. Appendix 3: Computer Programs Dealing with the Evaluation of DNA Sequence Variation and Molecular Marker Data. Appendix 4: Web Pages of Interest. References. Index....

Autorentext
Kurt Weising, Hilde Nybom, Markus Pfenninger, Kirsten Wolff, Günter Kahl

Klappentext
Like its successful predecessor, this completely revised second edition serves as a comprehensive introduction to the field, as well as a handy reference for the researcher. This edition focuses on both PCR-based fingerprinting and more sophisticated techniques. Supplying relevant background information, it provides a complete update of the extensive survey of applications of DNA fingerprinting in plants and a comparison of different types of molecular markers. This second edition is a valuable manual for beginners and active researchers.

Zusammenfassung
Focuses on PCR-based fingerprinting, including sophisticated techniques. This book provides a survey of applications of DNA fingerprinting in plants and a comparison of different types of molecular markers. It is suitable for beginners and active researchers.

Inhalt
Preface. Repetitive DNA: An Important Source of Variation in Eukaryotic Genomes. Detecting DNA Variation by Molecular Markers. Laboratory Equipment. Methodology. Evaluation of Molecular Marker Data. Applications of DNA Fingerprinting in Plant Sciences. Linkage Analysis and Genetic Maps. Which Marker for What Purpose: A Comparison. Future Prospects: SNiPs and Chips for DNA and RNA Profiling. Appendix 1: Plant DNA Isolation Protocols. Appendix 2: Suppliers and Sellers of Reagents and Equipment. Appendix 3: Computer Programs Dealing with the Evaluation of DNA Sequence Variation and Molecular Marker Data. Appendix 4: Web Pages of Interest. References. Index.

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