Label-Free Biosensors

Informationen zum Autor Dr Matthew A. Cooper is Founder and Managing Director of Cambridge Medical Innovations and Co-Founder and Chief Scientific Officer of Akubio. Dr Cooper is consulted widely for biosensor! biotechnology! and pharmaceutical companies in the UK! Europe! and the United States. He is a review panel member for BBSRC! EPSRC! US NIH! US NIAID! and Science Foundation Ireland and a Fellow of the Royal Society of Medicine. Klappentext A detailed technical review of label-free biosensor techniques with worked examples. Zusammenfassung Reviews both established and newer label-free techniques to give readers interested in the technologies and applications behind label-free an insight into the field from expert opinion leaders and practitioners of the technologies. Chapters contain worked examples that take readers through the basics of experimental design! setup and data analysis. Inhaltsverzeichnis 1. Label-free biosensors: an introduction Brian T. Cunningham; 2. Experimental design Robert Karlsson; 3. Extracting affinity constants from biosensor binding responses Rebecca L. Rich and David G. Myszka; 4. Extracting kinetic rate constants from binding responses Rebecca L. Rich and David G. Myszka; 5. Sensor surfaces and receptor deposition Matthew A. Cooper; 6. Macromolecular interactions Francis Markey; 7. Interactions with membranes and membrane receptors Matthew A. Cooper; 8. Application of SPR technology to pharmaceutical relevant drug-receptor interactions Walter Huber; 9. High-throughput analysis of biomolecular interactions and cellular responses with resonant waveguide grating biosensors Ye Fang! Jack Fang! Elizabeth Tran! Xinying Xie! Michael Hallstrom! and Anthony G. Frutos; 10. ITC-derived binding constants Richard K. Brown! Michael Brandts! Ronan O'Brien! and William B. Peters; 11. Impedance technology applied to cell-based assays Ryan McGuinness and Ed Verdonk. ...

Klappentext
A detailed technical review of label-free biosensor techniques with worked examples.

Zusammenfassung
Reviews both established and newer label-free techniques to give readers interested in the technologies and applications behind label-free an insight into the field from expert opinion leaders and practitioners of the technologies. Chapters contain worked examples that take readers through the basics of experimental design, setup and data analysis.

Inhalt
1. Label-free biosensors: an introduction Brian T. Cunningham; 2. Experimental design Robert Karlsson; 3. Extracting affinity constants from biosensor binding responses Rebecca L. Rich and David G. Myszka; 4. Extracting kinetic rate constants from binding responses Rebecca L. Rich and David G. Myszka; 5. Sensor surfaces and receptor deposition Matthew A. Cooper; 6. Macromolecular interactions Francis Markey; 7. Interactions with membranes and membrane receptors Matthew A. Cooper; 8. Application of SPR technology to pharmaceutical relevant drug-receptor interactions Walter Huber; 9. High-throughput analysis of biomolecular interactions and cellular responses with resonant waveguide grating biosensors Ye Fang, Jack Fang, Elizabeth Tran, Xinying Xie, Michael Hallstrom, and Anthony G. Frutos; 10. ITC-derived binding constants Richard K. Brown, Michael Brandts, Ronan O'Brien, and William B. Peters; 11. Impedance technology applied to cell-based assays Ryan McGuinness and Ed Verdonk.

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