Flow Cytometry

Flow cytometry is a technique widely used in biological research and in diagnostic medicine. Flow cytometers are found in most biological research institutions and most clinical laboratories in larger hospitals.

Flow Cytometry 3/e is intended as a handbook for every laboratory that has a bench-top flow cytometer or a fluorescence activated cell sorter. It is an introduction and guide to those new to the field and a first point of reference for experienced practitioners who want to investigate a new technique. The chapter on immunophenotyping - the most important clinical application of flow cytometry - has been strengthened by the addition of a chapter on quality control in the clinical laboratory. The utility of the book in a clinical laboratory has been further enhanced by the addition of a chapter covering ten other clinical applications. Flow cytometry has found increasing application in the field of apoptosis research. A new chapter has been added to cover this important topic. Every flow cytometry laboratory can't afford not to have a copy on the shelf as a first point of reference. The book is not fully comprehensive but it does aim to cover over 90% of the applications of flow cytometry in mammalian biology.

This book will be useful to those people who wish to begin exploring the fascinating and attractive world of flow cytometry and also to those who need to update their knowledge of flow cytometry protocols in mammalian cell biology. It is also an excellent reference book on flow cytometry for use in the laboratory.

Inhalt
Introduction to the principles of flow cytometry
Fluorescence and fluorescence technology
Preparing of suspensions of single cells
Flow sorting
Immunofluorescence of surface markers
Analysis of DNA - general methods
Further clinical applications
Quality control in the clinical laboratory
Measurement of cytoplasmic and nuclear antigens
Analysis of DNA-measurement of cell kinetics by the bromodeoxyuridine/anti-bromodeoxyuridine method
Analysis of cell proliferation using the bromodeoxyuridine/Hoechst-propidium iodide method
Chromosome analysis and sorting by flow cytometry
Intracellular ionised calcium, magnesium, membrane potential and pH
Flow cytometry in the study of apoptosis
Further applications to cell and molecular biology

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